8AIL
Bacillus phage VMY22 p56 in complex with Bacillus weidmannii Ung
Summary for 8AIL
| Entry DOI | 10.2210/pdb8ail/pdb |
| Descriptor | Uracil-DNA glycosylase, Bacillus phage VMY22 p56, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | inhibitor, complex, udg, ung, protein binding |
| Biological source | Bacillus wiedmannii More |
| Total number of polymer chains | 12 |
| Total formula weight | 159208.36 |
| Authors | Muselmani, W.,Bagneris, C.,Savva, R. (deposition date: 2022-07-26, release date: 2023-06-21, Last modification date: 2024-02-07) |
| Primary citation | Muselmani, W.,Kashif-Khan, N.,Bagneris, C.,Santangelo, R.,Williams, M.A.,Savva, R. A Multimodal Approach towards Genomic Identification of Protein Inhibitors of Uracil-DNA Glycosylase. Viruses, 15:-, 2023 Cited by PubMed Abstract: DNA-mimicking proteins encoded by viruses can modulate processes such as innate cellular immunity. An example is Ung-family uracil-DNA glycosylase inhibition, which prevents Ung-mediated degradation via the stoichiometric protein blockade of the Ung DNA-binding cleft. This is significant where uracil-DNA is a key determinant in the replication and distribution of virus genomes. Unrelated protein folds support a common physicochemical spatial strategy for Ung inhibition, characterised by pronounced sequence plasticity within the diverse fold families. That, and the fact that relatively few template sequences are biochemically verified to encode Ung inhibitor proteins, presents a barrier to the straightforward identification of Ung inhibitors in genomic sequences. In this study, distant homologs of known Ung inhibitors were characterised via structural biology and structure prediction methods. A recombinant cellular survival assay and in vitro biochemical assay were used to screen distant variants and mutants to further explore tolerated sequence plasticity in motifs supporting Ung inhibition. The resulting validated sequence repertoire defines an expanded set of heuristic sequence and biophysical signatures shared by known Ung inhibitor proteins. A computational search of genome database sequences and the results of recombinant tests of selected output sequences obtained are presented here. PubMed: 37376646DOI: 10.3390/v15061348 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.45 Å) |
Structure validation
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