8AFZ

Architecture of the ESCPE-1 membrane coat

8AFZ の概要

• エントリーDOI: 10.2210/pdb8afz/pdb
• EMDBエントリー: 15413
• 分子名称: Sorting nexin-1, Sorting nexin-5, Cation-independent mannose-6-phosphate receptor (3 entities in total)
• 機能のキーワード: sorting nexins, px domain, bar domain, endosomes, retrograde transport, endocytic recycling, cargo recognition, protein coat, membrane recruitment, membrane deformation, membrane tubules., transport protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 109801.88

構造登録者

Lopez-Robles, C.,Scaramuzza, S.,Astorga-Simon, E.,Ishida, M.,Williamsom, C.D.,Banos-Mateos, S.,Gil-Carton, D.,Romero, M.,Vidaurrazaga, A.,Fernandez-Recio, J.,Rojas, A.L.,Bonifacino, J.S.,Castano-Diez, D.,Hierro, A. (登録日: 2022-07-18, 公開日: 2023-06-21, 最終更新日: 2024-07-24)

主引用文献

Lopez-Robles, C.,Scaramuzza, S.,Astorga-Simon, E.N.,Ishida, M.,Williamson, C.D.,Banos-Mateos, S.,Gil-Carton, D.,Romero-Durana, M.,Vidaurrazaga, A.,Fernandez-Recio, J.,Rojas, A.L.,Bonifacino, J.S.,Castano-Diez, D.,Hierro, A.
Architecture of the ESCPE-1 membrane coat.
Nat.Struct.Mol.Biol., 30:958-969, 2023

PubMed Abstract: Recycling of membrane proteins enables the reuse of receptors, ion channels and transporters. A key component of the recycling machinery is the endosomal sorting complex for promoting exit 1 (ESCPE-1), which rescues transmembrane proteins from the endolysosomal pathway for transport to the trans-Golgi network and the plasma membrane. This rescue entails the formation of recycling tubules through ESCPE-1 recruitment, cargo capture, coat assembly and membrane sculpting by mechanisms that remain largely unknown. Herein, we show that ESCPE-1 has a single-layer coat organization and suggest how synergistic interactions between ESCPE-1 protomers, phosphoinositides and cargo molecules result in a global arrangement of amphipathic helices to drive tubule formation. Our results thus define a key process of tubule-based endosomal sorting.

PubMed: 37322239
DOI: 10.1038/s41594-023-01014-7

実験手法

ELECTRON MICROSCOPY (10 Å)