8AF9

Structure of a T4SS effector from Brucella sp.

8AF9 の概要

• エントリーDOI: 10.2210/pdb8af9/pdb
• 分子名称: NyxB, T4SS effector protein from Brucella (2 entities in total)
• 機能のキーワード: effector, bacteria, secretion system, toxin
• 由来する生物種: Brucella abortus
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 180634.82

構造登録者

Gueguen-Chaignon, V.,Salcedo, S.P.,Terradot, L. (登録日: 2022-07-16, 公開日: 2023-01-25, 最終更新日: 2024-06-19)

主引用文献

Louche, A.,Blanco, A.,Lacerda, T.L.S.,Cancade-Veyre, L.,Lionnet, C.,Berge, C.,Rolando, M.,Lembo, F.,Borg, J.P.,Buchrieser, C.,Nagahama, M.,Gerard, F.C.A.,Gorvel, J.P.,Gueguen-Chaignon, V.,Terradot, L.,Salcedo, S.P.
Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins.
Nat Commun, 14:102-102, 2023

PubMed Abstract: The cell nucleus is a primary target for intracellular bacterial pathogens to counteract immune responses and hijack host signalling pathways to cause disease. Here we identify two Brucella abortus effectors, NyxA and NyxB, that interfere with host protease SENP3, and this facilitates intracellular replication of the pathogen. The translocated Nyx effectors directly interact with SENP3 via a defined acidic patch (identified from the crystal structure of NyxB), preventing nucleolar localisation of SENP3 at late stages of infection. By sequestering SENP3, the effectors promote cytoplasmic accumulation of nucleolar AAA-ATPase NVL and ribosomal protein L5 (RPL5) in effector-enriched structures in the vicinity of replicating bacteria. The shuttling of ribosomal biogenesis-associated nucleolar proteins is inhibited by SENP3 and requires the autophagy-initiation protein Beclin1 and the SUMO-E3 ligase PIAS3. Our results highlight a nucleomodulatory function of two Brucella effectors and reveal that SENP3 is a crucial regulator of the subcellular localisation of nucleolar proteins during Brucella infection, promoting intracellular replication of the pathogen.

PubMed: 36609656
DOI: 10.1038/s41467-022-35763-8

実験手法

X-RAY DIFFRACTION (2.5 Å)