8AF8

Room temperature SSX crystal structure of CTX-M-14 (5K dataset)

8AF8 の概要

• エントリーDOI: 10.2210/pdb8af8/pdb
• 分子名称: Beta-lactamase, SULFATE ION (3 entities in total)
• 機能のキーワード: beta-lactamase, serial crystallography, ssx, tapedrive, hydrolase
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28075.46

構造登録者

Oberthuer, D.,Perbandt, M.,Prester, A.,Rohde, H.,Betzel, C.,Yefanov, O. (登録日: 2022-07-15, 公開日: 2022-11-16, 最終更新日: 2024-04-03)

主引用文献

Zielinski, K.A.,Prester, A.,Andaleeb, H.,Bui, S.,Yefanov, O.,Catapano, L.,Henkel, A.,Wiedorn, M.O.,Lorbeer, O.,Crosas, E.,Meyer, J.,Mariani, V.,Domaracky, M.,White, T.A.,Fleckenstein, H.,Sarrou, I.,Werner, N.,Betzel, C.,Rohde, H.,Aepfelbacher, M.,Chapman, H.N.,Perbandt, M.,Steiner, R.A.,Oberthuer, D.
Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
Iucrj, 9:778-791, 2022

PubMed Abstract: Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance ( CTX-M-14 β-lactamase, xylanase GH11 and urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

PubMed: 36381150
DOI: 10.1107/S2052252522010193

実験手法

X-RAY DIFFRACTION (1.55 Å)