8AC1

RNA polymerase at U-rich pause bound to non-regulatory RNA - inactive, open clamp state

8AC1 の概要

• エントリーDOI: 10.2210/pdb8ac1/pdb
• EMDBエントリー: 15330
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• 機能のキーワード: rna polymerase, transcriptional pausing, transcription termination, regulatory rna, transcription
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 611002.18

構造登録者

Dey, S.,Weixlbaumer, A. (登録日: 2022-07-05, 公開日: 2022-10-26, 最終更新日: 2024-07-24)

主引用文献

Dey, S.,Batisse, C.,Shukla, J.,Webster, M.W.,Takacs, M.,Saint-Andre, C.,Weixlbaumer, A.
Structural insights into RNA-mediated transcription regulation in bacteria.
Mol.Cell, 82:3885-, 2022

PubMed Abstract: RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at intrinsic terminators in all kingdoms of life. In contrast, the regulatory RNA putL suppresses pausing and termination in cis. However, how nascent RNA modulates its own synthesis remains largely unknown. We present cryo-EM reconstructions of RNAP captured during transcription of putL variants or an unrelated sequence at a U-rich pause site. Our results suggest how putL suppresses pausing and promotes its synthesis. We demonstrate that transcribing a U-rich sequence, a ubiquitous trigger of intrinsic termination, promotes widening of the RNAP nucleic-acid-binding channel. Widening destabilizes RNAP interactions with DNA and RNA to facilitate transcript dissociation reminiscent of intrinsic transcription termination. Surprisingly, RNAP remains bound to DNA after transcript release. Our results provide the structural framework to understand RNA-mediated intrinsic transcription termination.

PubMed: 36220101
DOI: 10.1016/j.molcel.2022.09.020

実験手法

ELECTRON MICROSCOPY (4.06 Å)