8AB0

Complex of RecO-RecR-DNA from Thermus thermophilus.

8AB0 の概要

• エントリーDOI: 10.2210/pdb8ab0/pdb
• 関連するPDBエントリー: 8A8J 8A93 8BPR
• EMDBエントリー: 15231 15267 15308 16164
• 分子名称: Recombination protein RecR, DNA repair protein RecO, Oligo1, ... (5 entities in total)
• 機能のキーワード: dna binding protein, dna repair pathway, recfor pathway, thermus thermophilus
• 由来する生物種: Thermus thermophilus HB8; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 130476.05

構造登録者

Nirwal, S.,Czarnocki-Cieciura, M.,Chaudhary, A.,Zajko, W.,Skowronek, K.,Chamera, S.,Figiel, M.,Nowotny, M. (登録日: 2022-07-04, 公開日: 2023-04-26, 最終更新日: 2024-07-24)

主引用文献

Nirwal, S.,Czarnocki-Cieciura, M.,Chaudhary, A.,Zajko, W.,Skowronek, K.,Chamera, S.,Figiel, M.,Nowotny, M.
Mechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination.
Nat.Struct.Mol.Biol., 30:650-660, 2023

PubMed Abstract: In bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They facilitate the replacement of SSB protein, which initially covers ssDNA, with RecA, which mediates the search for homologous sequences. However, the molecular mechanism of RecFOR cooperation remains largely unknown. We used Thermus thermophilus proteins to study this system. Here, we present a cryo-electron microscopy structure of the RecF-dsDNA complex, and another reconstruction that shows how RecF interacts with two different regions of the tetrameric RecR ring. Lower-resolution reconstructions of the RecR-RecO subcomplex and the RecFOR-DNA assembly explain how RecO is positioned to interact with ssDNA and SSB, which is proposed to lock the complex on a ssDNA-dsDNA junction. Our results integrate the biochemical data available for the RecFOR system and provide a framework for its complete understanding.

PubMed: 37081315
DOI: 10.1038/s41594-023-00967-z

実験手法

ELECTRON MICROSCOPY (6.09 Å)