8A8F

Crystal structure of Glc7 phosphatase in complex with the regulatory region of Ref2

8A8F の概要

• エントリーDOI: 10.2210/pdb8a8f/pdb
• 分子名称: Serine/threonine-protein phosphatase PP1-2, RNA end formation protein 2, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: ser/thr phosphatase, regulatory subunit, rna, transcription termination, gene regulation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44741.73

構造登録者

Carminati, M.,Manav, C.M.,Bellini, D.,Passmore, L.A. (登録日: 2022-06-22, 公開日: 2023-11-29, 最終更新日: 2024-06-12)

主引用文献

Carminati, M.,Rodriguez-Molina, J.B.,Manav, M.C.,Bellini, D.,Passmore, L.A.
A direct interaction between CPF and RNA Pol II links RNA 3' end processing to transcription.
Mol.Cell, 83:4461-4478.e13, 2023

PubMed Abstract: Transcription termination by RNA polymerase II (RNA Pol II) is linked to RNA 3' end processing by the cleavage and polyadenylation factor (CPF or CPSF). CPF contains endonuclease, poly(A) polymerase, and protein phosphatase activities, which cleave and polyadenylate pre-mRNAs and dephosphorylate RNA Pol II to control transcription. Exactly how the RNA 3' end processing machinery is coupled to transcription remains unclear. Here, we combine in vitro reconstitution, structural studies, and genome-wide analyses to show that yeast CPF physically and functionally interacts with RNA Pol II. Surprisingly, CPF-mediated dephosphorylation promotes the formation of an RNA Pol II stalk-to-stalk homodimer in vitro. This dimer is compatible with transcription but not with the binding of transcription elongation factors. Disruption of the dimerization interface in cells causes transcription defects, including altered RNA Pol II abundance on protein-coding genes, tRNA genes, and intergenic regions. We hypothesize that RNA Pol II dimerization may provide a mechanistic basis for the allosteric model of transcription termination.

PubMed: 38029752
DOI: 10.1016/j.molcel.2023.11.004

実験手法

X-RAY DIFFRACTION (1.85 Å)