8A4B

Structure of human Rep15:Rab3B_Q81L complex.

8A4B の概要

• エントリーDOI: 10.2210/pdb8a4b/pdb
• 分子名称: Rab15 effector protein, Ras-related protein Rab-3B, GUANOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: rab gtpase, effector, endocytosis
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 91661.15

構造登録者

Rai, A.,Vetter, I.R.,Goody, R.S. (登録日: 2022-06-10, 公開日: 2022-08-10, 最終更新日: 2024-11-06)

主引用文献

Rai, A.,Singh, A.K.,Bleimling, N.,Posern, G.,Vetter, I.R.,Goody, R.S.
Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector.
Nat Commun, 13:4262-4262, 2022

PubMed Abstract: In their GTP-bound (active) form, Rab proteins interact with effector proteins that control downstream signaling. One such Rab15 effector is Rep15, which is known to have a role in receptor recycling from the endocytic recycling compartment but otherwise remains poorly characterized. Here, we report the characterization of the Rep15:Rab15 interaction and identification of Rab3 paralogs and Rab34 as Rep15 interacting partners from a yeast two-hybrid assay. Biochemical validation of the interactions is presented and crystal structures of the Rep15:Rab3B and Rep15:Rab3C complexes provide additional mechanistic insight. We find that Rep15 adopts a globular structure that is distinct from other reported Rab15, Rab3 and Rab34 effectors. Structure-based mutagenesis experiments explain the Rep15:Rab interaction specificity. Rep15 depletion in U138MG glioblastoma cells impairs cell proliferation, cell migration and receptor recycling, underscoring the need for further clarification of the role of Rep15 in cancer.

PubMed: 35871249
DOI: 10.1038/s41467-022-31831-1

実験手法

X-RAY DIFFRACTION (2.8 Å)