8A3O

Structure of human Fy-4

8A3O の概要

• エントリーDOI: 10.2210/pdb8a3o/pdb
• 関連するPDBエントリー: 7ND2
• 分子名称: Quinone oxidoreductase-like protein 1 (2 entities in total)
• 機能のキーワード: ferry complex, mrna transport, early endosome, structural protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79322.83

構造登録者

Schuhmacher, J.S.,Zerial, M. (登録日: 2022-06-08, 公開日: 2022-06-29, 最終更新日: 2024-02-07)

主引用文献

Quentin, D.,Schuhmacher, J.S.,Klink, B.U.,Lauer, J.,Shaikh, T.R.,Huis In 't Veld, P.J.,Welp, L.M.,Urlaub, H.,Zerial, M.,Raunser, S.
Structural basis of mRNA binding by the human FERRY Rab5 effector complex.
Mol.Cell, 83:1856-1871.e9, 2023

PubMed Abstract: The pentameric FERRY Rab5 effector complex is a molecular link between mRNA and early endosomes in mRNA intracellular distribution. Here, we determine the cryo-EM structure of human FERRY. It reveals a unique clamp-like architecture that bears no resemblance to any known structure of Rab effectors. A combination of functional and mutational studies reveals that while the Fy-2 C-terminal coiled-coil acts as binding region for Fy-1/3 and Rab5, both coiled-coils and Fy-5 concur to bind mRNA. Mutations causing truncations of Fy-2 in patients with neurological disorders impair Rab5 binding or FERRY complex assembly. Thus, Fy-2 serves as a binding hub connecting all five complex subunits and mediating the binding to mRNA and early endosomes via Rab5. Our study provides mechanistic insights into long-distance mRNA transport and demonstrates that the particular architecture of FERRY is closely linked to a previously undescribed mode of RNA binding, involving coiled-coil domains.

PubMed: 37267906
DOI: 10.1016/j.molcel.2023.05.009

実験手法

X-RAY DIFFRACTION (2.9 Å)