8A3B

Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles

Summary for 8A3B

• Entry DOI: 10.2210/pdb8a3b/pdb
• EMDB information: 15110
• Descriptor: Pannexin-1 (1 entity in total)
• Functional Keywords: membrane transporter, atp-release channel, inflammation, immune function, membrane protein
• Biological source: Mus musculus (house mouse)
• Total number of polymer chains: 7
• Total formula weight: 353328.04

Authors

Drulyte, I. (deposition date: 2022-06-07, release date: 2023-02-08, Last modification date: 2024-07-24)

Primary citation

Drulyte, I.,Gutgsell, A.R.,Lloris-Garcera, P.,Liss, M.,Geschwindner, S.,Radjainia, M.,Frauenfeld, J.,Loving, R.
Direct cell extraction of membrane proteins for structure-function analysis.
Sci Rep, 13:1420-1420, 2023

PubMed Abstract: Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM.

PubMed: 36697499
DOI: 10.1038/s41598-023-28455-w

Experimental method

ELECTRON MICROSCOPY (3.1 Å)