7ZUT

Penicillium expansum chimera loop1

7ZUT の概要

• エントリーDOI: 10.2210/pdb7zut/pdb
• 関連するPDBエントリー: 7ZTF 7ZTJ
• 分子名称: Antifungal protein, CHLORIDE ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: penicillium expansum, antifungal protein, chimeric
• 由来する生物種: Penicillium expansum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6948.53

構造登録者

Gallego, F.,Marina, A.,Manzanares, P.,Marcos, J.F.,Giner Llorca, M. (登録日: 2022-05-13, 公開日: 2023-03-22, 最終更新日: 2024-11-06)

主引用文献

Giner-Llorca, M.,Del Sol, F.G.,Marcos, J.F.,Marina, A.,Manzanares, P.
Rationally designed antifungal protein chimeras reveal new insights into structure-activity relationship.
Int.J.Biol.Macromol., 225:135-148, 2023

PubMed Abstract: Antifungal proteins (AFPs) are promising antimicrobial compounds that represent a feasible alternative to fungicides. Penicillium expansum encodes three phylogenetically distinct AFPs (PeAfpA, PeAfpB and PeAfpC) which show different antifungal profiles and fruit protection effects. To gain knowledge about the structural determinants governing their activity, we solved the crystal structure of PeAfpB and rationally designed five PeAfpA::PeAfpB chimeras (chPeAFPV1-V5). Chimeras showed significant differences in their antifungal activity. chPeAFPV1 and chPeAFPV2 improved the parental PeAfpB potency, and it was very similar to that of PeAfpA. chPeAFPV4 and chPeAFPV5 showed an intermediate profile of activity compared to the parental proteins while chPeAFPV3 was inactive towards most of the fungi tested. Structural analysis of the chimeras evidenced an identical scaffold to PeAfpB, suggesting that the differences in activity are due to the contributions of specific residues and not to induced conformational changes or structural rearrangements. Results suggest that mannoproteins determine protein interaction with the cell wall and its antifungal activity while there is not a direct correlation between binding to membrane phospholipids and activity. This work provides new insights about the relevance of sequence motifs and the feasibility of modifying protein specificity, opening the door to the rational design of chimeras with biotechnological applicability.

PubMed: 36460243
DOI: 10.1016/j.ijbiomac.2022.11.280

実験手法

X-RAY DIFFRACTION (1.1 Å)