7ZS9

Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome (complex A)

7ZS9 の概要

• エントリーDOI: 10.2210/pdb7zs9/pdb
• EMDBエントリー: 14927
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (37 entities in total)
• 機能のキーワード: pic, nucleosome, tf, transcription
• 由来する生物種: Saccharomyces cerevisiae; 詳細
• タンパク質・核酸の鎖数: 38
• 化学式量合計: 1535673.52

構造登録者

Wang, H.,Cramer, P. (登録日: 2022-05-06, 公開日: 2022-11-16, 最終更新日: 2024-07-24)

主引用文献

Wang, H.,Schilbach, S.,Ninov, M.,Urlaub, H.,Cramer, P.
Structures of transcription preinitiation complex engaged with the +1 nucleosome.
Nat.Struct.Mol.Biol., 30:226-232, 2023

PubMed Abstract: The preinitiation complex (PIC) assembles on promoters of protein-coding genes to position RNA polymerase II (Pol II) for transcription initiation. Previous structural studies revealed the PIC on different promoters, but did not address how the PIC assembles within chromatin. In the yeast Saccharomyces cerevisiae, PIC assembly occurs adjacent to the +1 nucleosome that is located downstream of the core promoter. Here we present cryo-EM structures of the yeast PIC bound to promoter DNA and the +1 nucleosome located at three different positions. The general transcription factor TFIIH engages with the incoming downstream nucleosome and its translocase subunit Ssl2 (XPB in human TFIIH) drives the rotation of the +1 nucleosome leading to partial detachment of nucleosomal DNA and intimate interactions between TFIIH and the nucleosome. The structures provide insights into how transcription initiation can be influenced by the +1 nucleosome and may explain why the transcription start site is often located roughly 60 base pairs upstream of the dyad of the +1 nucleosome in yeast.

PubMed: 36411341
DOI: 10.1038/s41594-022-00865-w

実験手法

ELECTRON MICROSCOPY (3.1 Å)