7ZPQ

Structure of the RQT-bound 80S ribosome from S. cerevisiae (C1)

これはPDB形式変換不可エントリーです。

7ZPQ の概要

• エントリーDOI: 10.2210/pdb7zpq/pdb
• EMDBエントリー: 14861 15228 15280
• 分子名称: 18S ribosomal RNA, 40S ribosomal protein S4-A, Rps5p, ... (84 entities in total)
• 機能のキーワード: collision, rna binding, rqt, rqc, ribosome
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 82
• 化学式量合計: 3377263.45

構造登録者

Best, K.M.,Ikeuchi, K.,Kater, L.,Best, D.M.,Musial, J.,Matsuo, Y.,Berninghausen, O.,Becker, T.,Inada, T.,Beckmann, R. (登録日: 2022-04-28, 公開日: 2023-02-22, 最終更新日: 2024-07-24)

主引用文献

Best, K.,Ikeuchi, K.,Kater, L.,Best, D.,Musial, J.,Matsuo, Y.,Berninghausen, O.,Becker, T.,Inada, T.,Beckmann, R.
Structural basis for clearing of ribosome collisions by the RQT complex.
Nat Commun, 14:921-921, 2023

PubMed Abstract: Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality control pathways. Ribosome-associated quality control facilitates the degradation of incomplete translation products and requires dissociation of the stalled ribosomes. A central event is therefore the splitting of collided ribosomes by the ribosome quality control trigger complex, RQT, by an unknown mechanism. Here we show that RQT requires accessible mRNA and the presence of a neighboring ribosome. Cryogenic electron microscopy of RQT-ribosome complexes reveals that RQT engages the 40S subunit of the lead ribosome and can switch between two conformations. We propose that the Ski2-like helicase 1 (Slh1) subunit of RQT applies a pulling force on the mRNA, causing destabilizing conformational changes of the small ribosomal subunit, ultimately resulting in subunit dissociation. Our findings provide conceptual framework for a helicase-driven ribosomal splitting mechanism.

PubMed: 36801861
DOI: 10.1038/s41467-023-36230-8

実験手法

ELECTRON MICROSCOPY (3.47 Å)