7ZOW

Crystal structure of Synechocystis halorhodopsin (SyHR), Cl-pumping mode, O state

7ZOW の概要

• エントリーDOI: 10.2210/pdb7zow/pdb
• 分子名称: Synechocystis halorhodopsin, EICOSANE, OLEIC ACID, ... (5 entities in total)
• 機能のキーワード: rhodopsin, syhr, trapping, cryo, cryotrapping, o-state, k-state, sulfate, chloride, pump, anion, photocycle, ion transport, membrane protein
• 由来する生物種: Synechocystis sp. PCC 7509
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31770.53

構造登録者

Kovalev, K.,Bukhdruker, S.,Astashkin, R.,Vaganova, S.,Gordeliy, V. (登録日: 2022-04-26, 公開日: 2022-11-09, 最終更新日: 2024-01-31)

主引用文献

Astashkin, R.,Kovalev, K.,Bukhdruker, S.,Vaganova, S.,Kuzmin, A.,Alekseev, A.,Balandin, T.,Zabelskii, D.,Gushchin, I.,Royant, A.,Volkov, D.,Bourenkov, G.,Koonin, E.,Engelhard, M.,Bamberg, E.,Gordeliy, V.
Structural insights into light-driven anion pumping in cyanobacteria.
Nat Commun, 13:6460-6460, 2022

PubMed Abstract: Transmembrane ion transport is a key process in living cells. Active transport of ions is carried out by various ion transporters including microbial rhodopsins (MRs). MRs perform diverse functions such as active and passive ion transport, photo-sensing, and others. In particular, MRs can pump various monovalent ions like Na, K, Cl, I, NO. The only characterized MR proposed to pump sulfate in addition to halides belongs to the cyanobacterium Synechocystis sp. PCC 7509 and is named Synechocystis halorhodopsin (SyHR). The structural study of SyHR may help to understand what makes an MR pump divalent ions. Here we present the crystal structure of SyHR in the ground state, the structure of its sulfate-bound form as well as two photoreaction intermediates, the K and O states. These data reveal the molecular origin of the unique properties of the protein (exceptionally strong chloride binding and proposed pumping of divalent anions) and sheds light on the mechanism of anion release and uptake in cyanobacterial halorhodopsins. The unique properties of SyHR highlight its potential as an optogenetics tool and may help engineer different types of anion pumps with applications in optogenetics.

PubMed: 36309497
DOI: 10.1038/s41467-022-34019-9

実験手法

X-RAY DIFFRACTION (1.6 Å)