7ZOL
Cryo-EM structure of a CRISPR effector in complex with regulator
Summary for 7ZOL
| Entry DOI | 10.2210/pdb7zol/pdb |
| EMDB information | 14847 |
| Descriptor | Cas7-11, gRNA, TPR-CHAT, ... (4 entities in total) |
| Functional Keywords | crispr-cas, effector, regulator, crrna, guide rna, antiphage, antiviral protein |
| Biological source | Desulfonema magnum More |
| Total number of polymer chains | 3 |
| Total formula weight | 237369.79 |
| Authors | Babatunde, E.E.,Davide, T.,Bertrand, B.,Sergey, N.,Alexander, M.,Henning, S.,Dong, C.N. (deposition date: 2022-04-26, release date: 2022-11-30, Last modification date: 2024-07-24) |
| Primary citation | Ekundayo, B.,Torre, D.,Beckert, B.,Nazarov, S.,Myasnikov, A.,Stahlberg, H.,Ni, D. Structural insights into the regulation of Cas7-11 by TPR-CHAT. Nat.Struct.Mol.Biol., 30:135-139, 2023 Cited by PubMed Abstract: The CRISPR-guided caspase (Craspase) complex is an assembly of the target-specific RNA nuclease known as Cas7-11 bound to CRISPR RNA (crRNA) and an ancillary protein known as TPR-CHAT (tetratricopeptide repeats (TPR) fused with a CHAT domain). The Craspase complex holds promise as a tool for gene therapy and biomedical research, but its regulation is poorly understood. TPR-CHAT regulates Cas7-11 nuclease activity via an unknown mechanism. In the present study, we use cryoelectron microscopy to determine structures of the Desulfonema magnum (Dm) Craspase complex to gain mechanistic insights into its regulation. We show that DmTPR-CHAT stabilizes crRNA-bound DmCas7-11 in a closed conformation via a network of interactions mediated by the DmTPR-CHAT N-terminal domain, the DmCas7-11 insertion finger and Cas11-like domain, resulting in reduced target RNA accessibility and cleavage. PubMed: 36471056DOI: 10.1038/s41594-022-00894-5 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.03 Å) |
Structure validation
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