7ZOL

Cryo-EM structure of a CRISPR effector in complex with regulator

7ZOL の概要

• エントリーDOI: 10.2210/pdb7zol/pdb
• EMDBエントリー: 14847
• 分子名称: Cas7-11, gRNA, TPR-CHAT, ... (4 entities in total)
• 機能のキーワード: crispr-cas, effector, regulator, crrna, guide rna, antiphage, antiviral protein
• 由来する生物種: Desulfonema magnum; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 237369.79

構造登録者

Babatunde, E.E.,Davide, T.,Bertrand, B.,Sergey, N.,Alexander, M.,Henning, S.,Dong, C.N. (登録日: 2022-04-26, 公開日: 2022-11-30, 最終更新日: 2024-07-24)

主引用文献

Ekundayo, B.,Torre, D.,Beckert, B.,Nazarov, S.,Myasnikov, A.,Stahlberg, H.,Ni, D.
Structural insights into the regulation of Cas7-11 by TPR-CHAT.
Nat.Struct.Mol.Biol., 30:135-139, 2023

PubMed Abstract: The CRISPR-guided caspase (Craspase) complex is an assembly of the target-specific RNA nuclease known as Cas7-11 bound to CRISPR RNA (crRNA) and an ancillary protein known as TPR-CHAT (tetratricopeptide repeats (TPR) fused with a CHAT domain). The Craspase complex holds promise as a tool for gene therapy and biomedical research, but its regulation is poorly understood. TPR-CHAT regulates Cas7-11 nuclease activity via an unknown mechanism. In the present study, we use cryoelectron microscopy to determine structures of the Desulfonema magnum (Dm) Craspase complex to gain mechanistic insights into its regulation. We show that DmTPR-CHAT stabilizes crRNA-bound DmCas7-11 in a closed conformation via a network of interactions mediated by the DmTPR-CHAT N-terminal domain, the DmCas7-11 insertion finger and Cas11-like domain, resulting in reduced target RNA accessibility and cleavage.

PubMed: 36471056
DOI: 10.1038/s41594-022-00894-5

実験手法

ELECTRON MICROSCOPY (3.03 Å)