7ZNZ

Crystal structure of unliganded form of FucOB, a GH95 family alpha-1,2-fucosidase from Akkermansia muciniphila

Summary for 7ZNZ

• Entry DOI: 10.2210/pdb7znz/pdb
• Descriptor: FucOB, a GH95 family alpha-1,2-fucosidase, GLYCEROL (3 entities in total)
• Functional Keywords: gut microbiota glycosyl hydrolase fucosidase mucin bombay blood group blood conversion, hydrolase
• Biological source: Akkermansia muciniphila ATCC BAA-835
• Total number of polymer chains: 1
• Total formula weight: 84695.88

Authors

Anso, I.,Cifuente, J.O.,Trastoy, B.,Guerin, M.E. (deposition date: 2022-04-23, release date: 2023-04-19, Last modification date: 2024-02-07)

Primary citation

Anso, I.,Naegeli, A.,Cifuente, J.O.,Orrantia, A.,Andersson, E.,Zenarruzabeitia, O.,Moraleda-Montoya, A.,Garcia-Alija, M.,Corzana, F.,Del Orbe, R.A.,Borrego, F.,Trastoy, B.,Sjogren, J.,Guerin, M.E.
Turning universal O into rare Bombay type blood.
Nat Commun, 14:1765-1765, 2023

PubMed Abstract: Red blood cell antigens play critical roles in blood transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency in H antigen, the O Bombay phenotype, can only be transfused with group O blood to avoid serious transfusion reactions. We discover FucOB from the mucin-degrading bacteria Akkermansia muciniphila as an α-1,2-fucosidase able to hydrolyze Type I, Type II, Type III and Type V H antigens to obtain the afucosylated Bombay phenotype in vitro. X-ray crystal structures of FucOB show a three-domain architecture, including a GH95 glycoside hydrolase. The structural data together with site-directed mutagenesis, enzymatic activity and computational methods provide molecular insights into substrate specificity and catalysis. Furthermore, using agglutination tests and flow cytometry-based techniques, we demonstrate the ability of FucOB to convert universal O type into rare Bombay type blood, providing exciting possibilities to facilitate transfusion in recipients/patients with Bombay phenotype.

PubMed: 36997505
DOI: 10.1038/s41467-023-37324-z

Experimental method

X-RAY DIFFRACTION (1.8 Å)