7ZIN

JC Polyomavirus VP1 in complex with 6'-Sialyllactose glycomacromolecules (aliphatic linker)

これはPDB形式変換不可エントリーです。

7ZIN の概要

• エントリーDOI: 10.2210/pdb7zin/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900066
• 分子名称: Major capsid protein VP1, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose, ... (5 entities in total)
• 機能のキーワード: complex, sialic acid, capsid protein, viral protein
• 由来する生物種: JC polyomavirus
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 152614.36

構造登録者

Freytag, J.,Mueller, J.C.,Stehle, T. (登録日: 2022-04-08, 公開日: 2022-12-21, 最終更新日: 2024-01-31)

主引用文献

Konietzny, P.B.,Freytag, J.,Feldhof, M.I.,Muller, J.C.,Ohl, D.,Stehle, T.,Hartmann, L.
Synthesis of Homo- and Heteromultivalent Fucosylated and Sialylated Oligosaccharide Conjugates via Preactivated N -Methyloxyamine Precision Macromolecules and Their Binding to Polyomavirus Capsid Proteins.
Biomacromolecules, 23:5273-5284, 2022

PubMed Abstract: Glycoconjugates are a versatile class of bioactive molecules that have found application as vaccines and antivirals and in cancer therapy. Their synthesis typically involves elaborate functionalization and use of protecting groups on the carbohydrate component in order to ensure efficient and selective conjugation. Alternatively, non-functionalized, non-protected carbohydrates isolated from biological sources or derived through biotechnological methods can be directly conjugated -methyloxyamine groups. In this study, we introduce such -methyloxyamine groups into a variety of multivalent scaffolds─from small to oligomeric to polymeric scaffolds─making use of solid-phase polymer synthesis to assemble monodisperse sequence-defined macromolecules. These scaffolds are then successfully functionalized with different types of human milk oligosaccharides deriving a library of homo- and heteromultivalent glycoconjugates. Glycomacromolecules presenting oligosaccharide side chains with either α2,3- or α2,6-linked terminal sialic acid are used in a binding study with two types of polyomavirus capsid proteins showing that the multivalent presentation through the -methyloxyamine-derived scaffolds increases the number of contacts with the protein. Overall, a straightforward route to derive glycoconjugates from complex oligosaccharides with high variability yet control in the multivalent scaffold is presented, and applicability of the derived structures is demonstrated.

PubMed: 36398945
DOI: 10.1021/acs.biomac.2c01092

実験手法

X-RAY DIFFRACTION (1.648 Å)