7ZHR

Complex structure of drosophila Unr CSD789 and pAbp RRM3

Summary for 7ZHR

• Entry DOI: 10.2210/pdb7zhr/pdb
• Descriptor: Upstream of N-ras, isoform A, Polyadenylate-binding protein (3 entities in total)
• Functional Keywords: drosophila unr, csd, cold-shock domain, protein complex, ppi, drosophila pabp, rrm, unr, pabp, rna binding protein
• Biological source: Drosophila melanogaster (fruit fly); More
• Total number of polymer chains: 2
• Total formula weight: 36114.56

Authors

Hollmann, N.M.,Jagtap, P.K.A.,Hennig, J. (deposition date: 2022-04-07, release date: 2022-12-07, Last modification date: 2024-02-07)

Primary citation

Hollmann, N.M.,Jagtap, P.K.A.,Linse, J.B.,Ullmann, P.,Payr, M.,Murciano, B.,Simon, B.,Hub, J.S.,Hennig, J.
Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein.
Nucleic Acids Res., 51:1895-1913, 2023

PubMed Abstract: RNA binding proteins (RBPs) often engage multiple RNA binding domains (RBDs) to increase target specificity and affinity. However, the complexity of target recognition of multiple RBDs remains largely unexplored. Here we use Upstream of N-Ras (Unr), a multidomain RBP, to demonstrate how multiple RBDs orchestrate target specificity. A crystal structure of the three C-terminal RNA binding cold-shock domains (CSD) of Unr bound to a poly(A) sequence exemplifies how recognition goes beyond the classical ππ-stacking in CSDs. Further structural studies reveal several interaction surfaces between the N-terminal and C-terminal part of Unr with the poly(A)-binding protein (pAbp). All interactions are validated by mutational analyses and the high-resolution structures presented here will guide further studies to understand how both proteins act together in cellular processes.

PubMed: 36688322
DOI: 10.1093/nar/gkac1277

Experimental method

X-RAY DIFFRACTION (2.99 Å)