7ZGV

Serratia NucC bound to cA3

Summary for 7ZGV

• Entry DOI: 10.2210/pdb7zgv/pdb
• Descriptor: Serratia NucC, RNA (5'-R(P*AP*AP*A)-3'), CALCIUM ION, ... (5 entities in total)
• Functional Keywords: nuclease, rna binding protein
• Biological source: Serratia; More
• Total number of polymer chains: 2
• Total formula weight: 30037.61

Authors

Garcia-Doval, C.,Mayo-Munoz, D.,Smith, L.M.,Fineran, P.C. (deposition date: 2022-04-04, release date: 2022-10-26, Last modification date: 2024-02-07)

Primary citation

Mayo-Munoz, D.,Smith, L.M.,Garcia-Doval, C.,Malone, L.M.,Harding, K.R.,Jackson, S.A.,Hampton, H.G.,Fagerlund, R.D.,Gumy, L.F.,Fineran, P.C.
Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection.
Mol.Cell, 82:4471-4486.e9, 2022

PubMed Abstract: Bacteria have diverse defenses against phages. In response, jumbo phages evade multiple DNA-targeting defenses by protecting their DNA inside a nucleus-like structure. We previously demonstrated that RNA-targeting type III CRISPR-Cas systems provide jumbo phage immunity by recognizing viral mRNA exported from the nucleus for translation. Here, we demonstrate that recognition of phage mRNA by the type III system activates a cyclic triadenylate-dependent accessory nuclease, NucC. Although unable to access phage DNA in the nucleus, NucC degrades the bacterial chromosome, triggers cell death, and disrupts phage replication and maturation. Hence, type-III-mediated jumbo phage immunity occurs via abortive infection, with suppression of the viral epidemic protecting the population. We further show that type III systems targeting jumbo phages have diverse accessory nucleases, including RNases that provide immunity. Our study demonstrates how type III CRISPR-Cas systems overcome the inaccessibility of jumbo phage DNA to provide robust immunity.

PubMed: 36395770
DOI: 10.1016/j.molcel.2022.10.028

Experimental method

X-RAY DIFFRACTION (1.48 Å)