7ZGN

Plant/insect N-glycan active PNGase

Summary for 7ZGN

• Entry DOI: 10.2210/pdb7zgn/pdb
• Descriptor: Glpgli family protein (2 entities in total)
• Functional Keywords: plant n-glycans, pngase, gut microbiota, hydrolase
• Biological source: Phocaeicola massiliensis B84634 = Timone 84634 = DSM 17679 = JCM 13223
• Total number of polymer chains: 2
• Total formula weight: 124165.54

Authors

Basle, A.,Crouch, L.,Bolam, D. (deposition date: 2022-04-04, release date: 2022-09-07, Last modification date: 2024-11-20)

Primary citation

Crouch, L.I.,Urbanowicz, P.A.,Basle, A.,Cai, Z.P.,Liu, L.,Voglmeir, J.,Melo Diaz, J.M.,Benedict, S.T.,Spencer, D.I.R.,Bolam, D.N.
Plant N -glycan breakdown by human gut Bacteroides.
Proc.Natl.Acad.Sci.USA, 119:e2208168119-e2208168119, 2022

PubMed Abstract: The major nutrients available to the human colonic microbiota are complex glycans derived from the diet. To degrade this highly variable mix of sugar structures, gut microbes have acquired a huge array of different carbohydrate-active enzymes (CAZymes), predominantly glycoside hydrolases, many of which have specificities that can be exploited for a range of different applications. Plant -glycans are prevalent on proteins produced by plants and thus components of the diet, but the breakdown of these complex molecules by the gut microbiota has not been explored. Plant -glycans are also well characterized allergens in pollen and some plant-based foods, and when plants are used in heterologous protein production for medical applications, the -glycans present can pose a risk to therapeutic function and stability. Here we use a novel genome association approach for enzyme discovery to identify a breakdown pathway for plant complex -glycans encoded by a gut species and biochemically characterize five CAZymes involved, including structures of the PNGase and GH92 α-mannosidase. These enzymes provide a toolbox for the modification of plant -glycans for a range of potential applications. Furthermore, the keystone PNGase also has activity against insect-type -glycans, which we discuss from the perspective of insects as a nutrient source.

PubMed: 36122227
DOI: 10.1073/pnas.2208168119

Experimental method

X-RAY DIFFRACTION (1.95 Å)