7ZGN

Plant/insect N-glycan active PNGase

7ZGN の概要

• エントリーDOI: 10.2210/pdb7zgn/pdb
• 分子名称: Glpgli family protein (2 entities in total)
• 機能のキーワード: plant n-glycans, pngase, gut microbiota, hydrolase
• 由来する生物種: Phocaeicola massiliensis B84634 = Timone 84634 = DSM 17679 = JCM 13223
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 124165.54

構造登録者

Basle, A.,Crouch, L.,Bolam, D. (登録日: 2022-04-04, 公開日: 2022-09-07, 最終更新日: 2024-11-20)

主引用文献

Crouch, L.I.,Urbanowicz, P.A.,Basle, A.,Cai, Z.P.,Liu, L.,Voglmeir, J.,Melo Diaz, J.M.,Benedict, S.T.,Spencer, D.I.R.,Bolam, D.N.
Plant N -glycan breakdown by human gut Bacteroides.
Proc.Natl.Acad.Sci.USA, 119:e2208168119-e2208168119, 2022

PubMed Abstract: The major nutrients available to the human colonic microbiota are complex glycans derived from the diet. To degrade this highly variable mix of sugar structures, gut microbes have acquired a huge array of different carbohydrate-active enzymes (CAZymes), predominantly glycoside hydrolases, many of which have specificities that can be exploited for a range of different applications. Plant -glycans are prevalent on proteins produced by plants and thus components of the diet, but the breakdown of these complex molecules by the gut microbiota has not been explored. Plant -glycans are also well characterized allergens in pollen and some plant-based foods, and when plants are used in heterologous protein production for medical applications, the -glycans present can pose a risk to therapeutic function and stability. Here we use a novel genome association approach for enzyme discovery to identify a breakdown pathway for plant complex -glycans encoded by a gut species and biochemically characterize five CAZymes involved, including structures of the PNGase and GH92 α-mannosidase. These enzymes provide a toolbox for the modification of plant -glycans for a range of potential applications. Furthermore, the keystone PNGase also has activity against insect-type -glycans, which we discuss from the perspective of insects as a nutrient source.

PubMed: 36122227
DOI: 10.1073/pnas.2208168119

実験手法

X-RAY DIFFRACTION (1.95 Å)