7ZF8

SARS-CoV-2 Omicron BA.2 RBD in complex with COVOX-150 Fab

7ZF8 の概要

• エントリーDOI: 10.2210/pdb7zf8/pdb
• 関連するPDBエントリー: 7ZF3 7ZF4 7ZF5 7ZF6
• 分子名称: COVOX-150 heavy chain, COVOX-150 light chain, Spike protein S1 (3 entities in total)
• 機能のキーワード: sars-cov-2, omicron, ba.1, ba.2, rbd, antibody, fab, covox-150, viral protein/immune system, viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69901.36

構造登録者

Zhou, D.,Huo, J.,Ren, J.,Stuart, D.I. (登録日: 2022-04-01, 公開日: 2022-06-01, 最終更新日: 2024-10-16)

主引用文献

Nutalai, R.,Zhou, D.,Tuekprakhon, A.,Ginn, H.M.,Supasa, P.,Liu, C.,Huo, J.,Mentzer, A.J.,Duyvesteyn, H.M.E.,Dijokaite-Guraliuc, A.,Skelly, D.,Ritter, T.G.,Amini, A.,Bibi, S.,Adele, S.,Johnson, S.A.,Constantinides, B.,Webster, H.,Temperton, N.,Klenerman, P.,Barnes, E.,Dunachie, S.J.,Crook, D.,Pollard, A.J.,Lambe, T.,Goulder, P.,Paterson, N.G.,Williams, M.A.,Hall, D.R.,Mongkolsapaya, J.,Fry, E.E.,Dejnirattisai, W.,Ren, J.,Stuart, D.I.,Screaton, G.R.
Potent cross-reactive antibodies following Omicron breakthrough in vaccinees.
Cell, 185:2116-2131.e18, 2022

PubMed Abstract: Highly transmissible Omicron variants of SARS-CoV-2 currently dominate globally. Here, we compare neutralization of Omicron BA.1, BA.1.1, and BA.2. BA.2 RBD has slightly higher ACE2 affinity than BA.1 and slightly reduced neutralization by vaccine serum, possibly associated with its increased transmissibility. Neutralization differences between sub-lineages for mAbs (including therapeutics) mostly arise from variation in residues bordering the ACE2 binding site; however, more distant mutations S371F (BA.2) and R346K (BA.1.1) markedly reduce neutralization by therapeutic antibody Vir-S309. In-depth structure-and-function analyses of 27 potent RBD-binding mAbs isolated from vaccinated volunteers following breakthrough Omicron-BA.1 infection reveals that they are focused in two main clusters within the RBD, with potent right-shoulder antibodies showing increased prevalence. Selection and somatic maturation have optimized antibody potency in less-mutated epitopes and recovered potency in highly mutated epitopes. All 27 mAbs potently neutralize early pandemic strains, and many show broad reactivity with variants of concern.

PubMed: 35662412
DOI: 10.1016/j.cell.2022.05.014

実験手法

X-RAY DIFFRACTION (2.95 Å)