7ZA4

GSTF sh155 mutant

7ZA4 の概要

• エントリーDOI: 10.2210/pdb7za4/pdb
• 分子名称: Glutathione transferase, SODIUM ION (3 entities in total)
• 機能のキーワード: glutathione, detoxification, xenobiotics, herbicides, transferase
• 由来する生物種: Alopecurus myosuroides
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 77216.90

構造登録者

Papageorgiou, A.C. (登録日: 2022-03-22, 公開日: 2022-07-20, 最終更新日: 2024-01-31)

主引用文献

Ioannou, E.,Papageorgiou, A.C.,Labrou, N.E.
Directed Evolution of Phi Class Glutathione Transferases Involved in Multiple-Herbicide Resistance of Grass Weeds and Crops.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: The extensive application of herbicides in crop cultivation has indisputably led to the emergence of weed populations characterized by multiple herbicide resistance (MHR). This phenomenon is associated with the enhanced metabolism and detoxifying ability of endogenous enzymes, such as phi class glutathione transferases (GSTFs). In the present work, a library of mutant GSTFs was created by in vitro directed evolution via DNA shuffling. Selected genes from the weeds and , and the cereal crops and were recombined to forge a library of novel chimeric GSTFs. The library was activity screened and the best-performing enzyme variants were purified and characterized. The work allowed the identification of enzyme variants that exhibit an eight-fold improvement in their catalytic efficiency, higher thermal stability (8.3 °C) and three-times higher inhibition sensitivity towards the herbicide butachlor. The crystal structures of the best-performing enzyme variants were determined by X-ray crystallography. Structural analysis allowed the identification of specific structural elements that are responsible for k regulation, thermal stability and inhibition potency. These improved novel enzymes hold the potential for utilization in biocatalysis and green biotechnology applications. The results of the present work contribute significantly to our knowledge of the structure and function of phi class plant GSTs and shed light on their involvement in the mechanisms of MHR.

PubMed: 35806486
DOI: 10.3390/ijms23137469

実験手法

X-RAY DIFFRACTION (2.05 Å)