7Z8K

Cytoplasmic dynein (A1) bound to BICDR1

7Z8K の概要

• エントリーDOI: 10.2210/pdb7z8k/pdb
• EMDBエントリー: 14555
• 分子名称: Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein 1 light intermediate chain 2, Cytoplasmic dynein 1 intermediate chain 2, ... (5 entities in total)
• 機能のキーワード: dynein, dynactin, cargo transport, activating adaptor, cytoskeleton, structural protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 1487639.21

構造登録者

Chaaban, S.,Carter, A.P. (登録日: 2022-03-17, 公開日: 2022-07-27, 最終更新日: 2024-07-24)

主引用文献

Chaaban, S.,Carter, A.P.
Structure of dynein-dynactin on microtubules shows tandem adaptor binding.
Nature, 610:212-216, 2022

PubMed Abstract: Cytoplasmic dynein is a microtubule motor that is activated by its cofactor dynactin and a coiled-coil cargo adaptor. Up to two dynein dimers can be recruited per dynactin, and interactions between them affect their combined motile behaviour. Different coiled-coil adaptors are linked to different cargos, and some share motifs known to contact sites on dynein and dynactin. There is limited structural information on how the resulting complex interacts with microtubules and how adaptors are recruited. Here we develop a cryo-electron microscopy processing pipeline to solve the high-resolution structure of dynein-dynactin and the adaptor BICDR1 bound to microtubules. This reveals the asymmetric interactions between neighbouring dynein motor domains and how they relate to motile behaviour. We found that two adaptors occupy the complex. Both adaptors make similar interactions with the dyneins but diverge in their contacts with each other and dynactin. Our structure has implications for the stability and stoichiometry of motor recruitment by cargos.

PubMed: 36071160
DOI: 10.1038/s41586-022-05186-y

実験手法

ELECTRON MICROSCOPY (4.37 Å)