Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

7Z6E

Structure of the C1-PH-CNH regulatory module of MRCK1

7Z6E の概要
エントリーDOI10.2210/pdb7z6e/pdb
分子名称Serine/threonine-protein kinase mrck-1, ZINC ION (3 entities in total)
機能のキーワードkinase, membrane, beta propeller, ph, lipid binding protein
由来する生物種Caenorhabditis elegans
タンパク質・核酸の鎖数5
化学式量合計327403.29
構造登録者
Truebestein, L.,Leonard, T.A. (登録日: 2022-03-11, 公開日: 2023-02-22, 最終更新日: 2024-05-01)
主引用文献Truebestein, L.,Antonioli, S.,Waltenberger, E.,Gehin, C.,Gavin, A.C.,Leonard, T.A.
Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase.
Structure, 31:435-, 2023
Cited by
PubMed Abstract: Protein kinases of the dystonia myotonica protein kinase (DMPK) family are critical regulators of actomyosin contractility in cells. The DMPK kinase MRCK1 is required for the activation of myosin, leading to the development of cortical tension, apical constriction, and early gastrulation. Here, we present the structure, conformation, and membrane-binding properties of Caenorhabditis elegans MRCK1. MRCK1 forms a homodimer with N-terminal kinase domains, a parallel coiled coil of 55 nm, and a C-terminal tripartite module of C1, pleckstrin homology (PH), and citron homology (CNH) domains. We report the high-resolution structure of the membrane-binding C1-PH-CNH module of MRCK1 and, using high-throughput and conventional liposome-binding assays, determine its binding to specific phospholipids. We further characterize the interaction of the C-terminal CRIB motif with Cdc42. The length of the coiled-coil domain of DMPK kinases is remarkably conserved over millions of years of evolution, suggesting that they may function as molecular rulers to position kinase activity at a fixed distance from the membrane.
PubMed: 36854301
DOI: 10.1016/j.str.2023.02.002
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.14 Å)
構造検証レポート
Validation report summary of 7z6e
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

PDB statisticsPDBj update infoContact PDBjnumon