7Z5E

VP2-only capsid of MVM D263A mutant

これはPDB形式変換不可エントリーです。

7Z5E の概要

• エントリーDOI: 10.2210/pdb7z5e/pdb
• EMDBエントリー: 14520
• 分子名称: Capsid protein VP1 (1 entity in total)
• 機能のキーワード: capsid, mvm, vlp, virus like particle
• 由来する生物種: Minute virus of mice
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 64558.46

構造登録者

Luque, D.,Ortega-Esteban, A.,Valbuena, A.,Vilas, J.L.,Rodriguez-Huete, A.,Mateu, M.G.,Caston, J.R. (登録日: 2022-03-09, 公開日: 2023-03-08, 最終更新日: 2024-07-17)

主引用文献

Luque, D.,Ortega-Esteban, A.,Valbuena, A.,Luis Vilas, J.,Rodriguez-Huete, A.,Mateu, M.G.,Caston, J.R.
Equilibrium Dynamics of a Biomolecular Complex Analyzed at Single-amino Acid Resolution by Cryo-electron Microscopy.
J.Mol.Biol., 435:168024-168024, 2023

PubMed Abstract: The biological function of macromolecular complexes depends not only on large-scale transitions between conformations, but also on small-scale conformational fluctuations at equilibrium. Information on the equilibrium dynamics of biomolecular complexes could, in principle, be obtained from local resolution (LR) data in cryo-electron microscopy (cryo-EM) maps. However, this possibility had not been validated by comparing, for a same biomolecular complex, LR data with quantitative information on equilibrium dynamics obtained by an established solution technique. In this study we determined the cryo-EM structure of the minute virus of mice (MVM) capsid as a model biomolecular complex. The LR values obtained correlated with crystallographic B factors and with hydrogen/deuterium exchange (HDX) rates obtained by mass spectrometry (HDX-MS), a gold standard for determining equilibrium dynamics in solution. This result validated a LR-based cryo-EM approach to investigate, with high spatial resolution, the equilibrium dynamics of biomolecular complexes. As an application of this approach, we determined the cryo-EM structure of two mutant MVM capsids and compared their equilibrium dynamics with that of the wild-type MVM capsid. The results supported a previously suggested linkage between mechanical stiffening and impaired equilibrium dynamics of a virus particle. Cryo-EM is emerging as a powerful approach for simultaneously acquiring information on the atomic structure and local equilibrium dynamics of biomolecular complexes.

PubMed: 36828271
DOI: 10.1016/j.jmb.2023.168024

実験手法

ELECTRON MICROSCOPY (3.32 Å)