7Z0F

CPAP:S-TUBULIN:IIH5 ALPHAREP COMPLEX

7Z0F の概要

• エントリーDOI: 10.2210/pdb7z0f/pdb
• 関連するPDBエントリー: 7Q1E 7Q1F 7Z0G
• 分子名称: Tubulin alpha chain, Tubulin beta chain, IIH5 ALPHAREP, ... (9 entities in total)
• 機能のキーワード: microtubule, vinca domain inhibitors, centriole, cell cycle
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129353.30

構造登録者

Gigant, B.,Campanacci, V. (登録日: 2022-02-22, 公開日: 2022-04-13, 最終更新日: 2024-01-31)

主引用文献

Campanacci, V.,Urvoas, A.,Ammar Khodja, L.,Aumont-Nicaise, M.,Noiray, M.,Lachkar, S.,Curmi, P.A.,Minard, P.,Gigant, B.
Structural convergence for tubulin binding of CPAP and vinca domain microtubule inhibitors.
Proc.Natl.Acad.Sci.USA, 119:e2120098119-e2120098119, 2022

PubMed Abstract: Microtubule dynamics is regulated by various cellular proteins and perturbed by small-molecule compounds. To what extent the mechanism of the former resembles that of the latter is an open question. We report here structures of tubulin bound to the PN2-3 domain of CPAP, a protein controlling the length of the centrioles. We show that an α-helix of the PN2-3 N-terminal region binds and caps the longitudinal surface of the tubulin β subunit. Moreover, a PN2-3 N-terminal stretch lies in a β-tubulin site also targeted by fungal and bacterial peptide-like inhibitors of the vinca domain, sharing a very similar binding mode with these compounds. Therefore, our results identify several characteristic features of cellular partners that bind to this site and highlight a structural convergence of CPAP with small-molecule inhibitors of microtubule assembly.

PubMed: 35507869
DOI: 10.1073/pnas.2120098119

実験手法

X-RAY DIFFRACTION (2.396 Å)