7YZV

Ryegrass mottle virus serine protease domain S159A mutant

7YZV の概要

• エントリーDOI: 10.2210/pdb7yzv/pdb
• 関連するPDBエントリー: 6FEZ 6FF0
• 分子名称: RNA-directed RNA polymerase (2 entities in total)
• 機能のキーワード: serine protease, viral protease, viral polypeptide, viral genome-linked protein, viral protein
• 由来する生物種: Ryegrass mottle virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20867.98

構造登録者

Kalnins, G. (登録日: 2022-02-21, 公開日: 2022-03-02, 最終更新日: 2024-11-06)

主引用文献

Kalnins, G.,Ludviga, R.,Kalnciema, I.,Resevica, G.,Zeltina, V.,Bogans, J.,Tars, K.,Zeltins, A.,Balke, I.
VPg Impact on Ryegrass Mottle Virus Serine-like 3C Protease Proteolysis and Structure.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: Sobemoviruses encode serine-like 3C proteases (Pro) that participate in the processing and maturation of other virus-encoded proteins. Its and activity is mediated by the naturally unfolded virus-genome-linked protein (VPg). Nuclear magnetic resonance studies show a Pro-VPg complex interaction and VPg tertiary structure; however, information regarding structural changes of the Pro-VPg complex during interaction is lacking. Here, we solved a full Pro-VPg 3D structure of ryegrass mottle virus (RGMoV) that demonstrates the structural changes in three different conformations due to VPg interaction with Pro. We identified a unique site of VPg interaction with Pro that was not observed in other sobemoviruses, and observed different conformations of the Pro β2 barrel. This is the first report of a full plant Pro crystal structure with its VPg cofactor. We also confirmed the existence of an unusual previously unmapped cleavage site for sobemovirus Pro in the transmembrane domain: E/A. We demonstrated that RGMoV Pro activity is not regulated by VPg and that , VPg can also mediate Pro in free form. Additionally, we observed Ca and Zn inhibitory effects on the Pro cleavage activity.

PubMed: 36982419
DOI: 10.3390/ijms24065347

実験手法

X-RAY DIFFRACTION (1.6 Å)