7YYO

Cryo-EM structure of an a-carboxysome RuBisCO enzyme at 2.9 A resolution

7YYO の概要

• エントリーDOI: 10.2210/pdb7yyo/pdb
• EMDBエントリー: 14385
• 分子名称: Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: carboxysome, carbon fixation, cyanobacteria, photosynthesis
• 由来する生物種: Cyanobium; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 526996.50

構造登録者

Mann, D.,Evans, S.L.,Bergeron, J.R.C. (登録日: 2022-02-18, 公開日: 2023-01-25, 最終更新日: 2025-10-01)

主引用文献

Evans, S.L.,Al-Hazeem, M.M.J.,Mann, D.,Smetacek, N.,Beavil, A.J.,Sun, Y.,Chen, T.,Dykes, G.F.,Liu, L.N.,Bergeron, J.R.C.
Single-particle cryo-EM analysis of the shell architecture and internal organization of an intact alpha-carboxysome.
Structure, 31:677-, 2023

PubMed Abstract: Carboxysomes are proteinaceous bacterial microcompartments that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating several enzymes, including ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for the first step of the Calvin-Benson-Bassham cycle. Despite their significance in carbon fixation and great bioengineering potentials, the structural understanding of native carboxysomes is currently limited to low-resolution studies. Here, we report the characterization of a native α-carboxysome from a marine cyanobacterium by single-particle cryoelectron microscopy (cryo-EM). We have determined the structure of its RuBisCO enzyme, and obtained low-resolution maps of its icosahedral shell, and of its concentric interior organization. Using integrative modeling approaches, we have proposed a complete atomic model of an intact carboxysome, providing insight into its organization and assembly. This is critical for a better understanding of the carbon fixation mechanism and toward repurposing carboxysomes in synthetic biology for biotechnological applications.

PubMed: 37015227
DOI: 10.1016/j.str.2023.03.008

実験手法

ELECTRON MICROSCOPY (2.87 Å)