7YV3

genetically encoded pH sensor Lime at pH10

7YV3 の概要

• エントリーDOI: 10.2210/pdb7yv3/pdb
• 分子名称: Lime (2 entities in total)
• 機能のキーワード: ph sensor, genetically encoded, florescent protein, fluorescent protein
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26812.08

構造登録者

Wen, Y.,Shen, Y.,Campbell, R.,Lemieux, M.J. (登録日: 2022-08-18, 公開日: 2023-08-16, 最終更新日: 2023-11-15)

主引用文献

Shen, Y.,Wen, Y.,Sposini, S.,Vishwanath, A.A.,Abdelfattah, A.S.,Schreiter, E.R.,Lemieux, M.J.,de Juan-Sanz, J.,Perrais, D.,Campbell, R.E.
Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin.
ACS Sens, 8:3014-3022, 2023

PubMed Abstract: Genetically encoded pH sensors based on fluorescent proteins are valuable tools for the imaging of cellular events that are associated with pH changes, such as exocytosis and endocytosis. Superecliptic pHluorin (SEP) is a pH-sensitive green fluorescent protein (GFP) variant widely used for such applications. Here, we report the rational design, development, structure, and applications of Lime, an improved SEP variant with higher fluorescence brightness and greater pH sensitivity. The X-ray crystal structure of Lime supports the mechanistic rationale that guided the introduction of beneficial mutations. Lime provides substantial improvements relative to SEP for imaging of endocytosis and exocytosis. Furthermore, Lime and its variants are advantageous for a broader range of applications including the detection of synaptic release and neuronal voltage changes.

PubMed: 37481776
DOI: 10.1021/acssensors.3c00484

実験手法

X-RAY DIFFRACTION (2 Å)