7YP9

Cryo-EM structure of Escherichia coli paused complex of transcription termination (TTC-pause)

Summary for 7YP9

• Entry DOI: 10.2210/pdb7yp9/pdb
• EMDB information: 33996
• Descriptor: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• Functional Keywords: termination, intrinsic termination, u-tract pause, transcription pause, factor-independent termination, transcription-dna-rna complex, transcription/dna/rna
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 8
• Total formula weight: 416394.07

Authors

You, L.L.,Zhang, Y. (deposition date: 2022-08-03, release date: 2022-11-16, Last modification date: 2024-07-03)

Primary citation

You, L.,Omollo, E.O.,Yu, C.,Mooney, R.A.,Shi, J.,Shen, L.,Wu, X.,Wen, A.,He, D.,Zeng, Y.,Feng, Y.,Landick, R.,Zhang, Y.
Structural basis for intrinsic transcription termination.
Nature, 613:783-789, 2023

PubMed Abstract: Efficient and accurate termination is required for gene transcription in all living organisms. Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway-called intrinsic termination transcription in bacteria-in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of Escherichia coli transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.

PubMed: 36631609
DOI: 10.1038/s41586-022-05604-1

Experimental method

ELECTRON MICROSCOPY (3.58 Å)