7YOB

Crystal structure of Aldehyde dehydrogenase 1A1 from mouse

7YOB の概要

• エントリーDOI: 10.2210/pdb7yob/pdb
• 分子名称: Aldehyde dehydrogenase 1A1 (1 entity in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 442907.82

構造登録者

Zhang, X.Y.,Ouyang, Z.Q. (登録日: 2022-08-01, 公開日: 2022-08-17, 最終更新日: 2023-11-29)

主引用文献

Zhang, X.,Ouyang, Z.
Crystal structure of aldehyde dehydrogenase 1A1 from mouse.
Biochem.Biophys.Res.Commun., 628:141-146, 2022

PubMed Abstract: Aldehyde dehydrogenase 1A1 (ALDH1A1) is an enzyme that catalyzes the NAD-dependent oxidation of aldehydes to carboxylic acids, participating in various metabolic processes. Currently, only structures from human and Ovis aries have been reported. Here we show a 2.89 Å resolution structure of ALDH1A1 from mice using X-ray crystallography. We performed a detailed analysis of the structure and compared it with ALDH1A1 structures from two other species, highlighting the significance of the differences. Structural superimposition reveals that the tetrameric molecule is asymmetrical, and the NAD-binding domain exhibits a certain rotation. In addition, the noticeable structural differences were detected, including the unique contact between Ser461 and Asp148, as well as the side chain orientations of three amino acids residues, Asn474, Met471 and Phe466. This study helps to expand the structural diversity of the ALDH family.

PubMed: 36084552
DOI: 10.1016/j.bbrc.2022.08.054

実験手法

X-RAY DIFFRACTION (2.89 Å)