7YMT
Cryo-EM structure of MERS-CoV spike protein, Two RBD-up conformation 2
Summary for 7YMT
| Entry DOI | 10.2210/pdb7ymt/pdb |
| EMDB information | 33942 |
| Descriptor | Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total) |
| Functional Keywords | mers-cov, spike, glycoprotein, viral protein |
| Biological source | Human betacoronavirus 2c EMC/2012 |
| Total number of polymer chains | 3 |
| Total formula weight | 462905.54 |
| Authors | Hsu, S.T.D.,Chang, N.E.,Weng, Z.W.,Yang, T.J.,Draczkowski, P. (deposition date: 2022-07-29, release date: 2023-08-09, Last modification date: 2024-11-13) |
| Primary citation | Tsai, Y.X.,Chang, N.E.,Reuter, K.,Chang, H.T.,Yang, T.J.,von Bulow, S.,Sehrawat, V.,Zerrouki, N.,Tuffery, M.,Gecht, M.,Grothaus, I.L.,Colombi Ciacchi, L.,Wang, Y.S.,Hsu, M.F.,Khoo, K.H.,Hummer, G.,Hsu, S.D.,Hanus, C.,Sikora, M. Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Cell, 187:1296-1311.e26, 2024 Cited by PubMed Abstract: Most membrane proteins are modified by covalent addition of complex sugars through N- and O-glycosylation. Unlike proteins, glycans do not typically adopt specific secondary structures and remain very mobile, shielding potentially large fractions of protein surface. High glycan conformational freedom hinders complete structural elucidation of glycoproteins. Computer simulations may be used to model glycosylated proteins but require hundreds of thousands of computing hours on supercomputers, thus limiting routine use. Here, we describe GlycoSHIELD, a reductionist method that can be implemented on personal computers to graft realistic ensembles of glycan conformers onto static protein structures in minutes. Using molecular dynamics simulation, small-angle X-ray scattering, cryoelectron microscopy, and mass spectrometry, we show that this open-access toolkit provides enhanced models of glycoprotein structures. Focusing on N-cadherin, human coronavirus spike proteins, and gamma-aminobutyric acid receptors, we show that GlycoSHIELD can shed light on the impact of glycans on the conformation and activity of complex glycoproteins. PubMed: 38428397DOI: 10.1016/j.cell.2024.01.034 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (6.55 Å) |
Structure validation
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