7YMO

Crystal structure of the recombination mediator protein RecO from Campylobacter jejuni

7YMO の概要

• エントリーDOI: 10.2210/pdb7ymo/pdb
• 分子名称: Recombination protein RecO, ZINC ION (3 entities in total)
• 機能のキーワード: recombination mediator protein, recombination
• 由来する生物種: Campylobacter jejuni
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25126.54

構造登録者

Lee, S.,Oh, H.B.,Yoon, S.I. (登録日: 2022-07-29, 公開日: 2022-08-31, 最終更新日: 2024-05-29)

主引用文献

Lee, S.J.,Oh, H.B.,Yoon, S.I.
Crystal Structure of the Recombination Mediator Protein RecO from Campylobacter jejuni and Its Interaction with DNA and a Zinc Ion.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: Homologous recombination is involved in repairing DNA damage, contributing to maintaining the integrity and stability of viral and cellular genomes. In bacteria, the recombination mediator proteins RecO and RecR are required to load the RecA recombinase on ssDNA for homologous recombination. To structurally and functionally characterize RecO, we determined the crystal structure of RecO from (cjRecO) at a 1.8 Å resolution and biochemically assessed its capacity to interact with DNA and a metal ion. cjRecO folds into a curved rod-like structure that consists of an N-terminal domain (NTD), C-terminal domain (CTD), and Zn-binding domain (ZnD). The ZnD at the end of the rod-like structure coordinates three cysteine residues and one histidine residue to accommodate a Zn ion. Based on an extensive comparative analysis of RecO structures and sequences, we propose that the Zn-binding consensus sequence of RecO is CxxC…C/HxxC/H/D. The interaction with Zn is indispensable for the protein stability of cjRecO but does not seem to be required for the recombination mediator function. cjRecO also interacts with ssDNA as part of its biological function, potentially using the positively charged patch in the NTD and CTD. However, cjRecO displays a low ssDNA-binding affinity, suggesting that cjRecO requires RecR to efficiently recognize ssDNA for homologous recombination.

PubMed: 36077065
DOI: 10.3390/ijms23179667

実験手法

X-RAY DIFFRACTION (1.8 Å)