7YEU

Superfolder green fluorescent protein with phosphine unnatural amino acid P3BF

7YEU の概要

• エントリーDOI: 10.2210/pdb7yeu/pdb
• 分子名称: Superfolder green fluorescent protein (2 entities in total)
• 機能のキーワード: unnatural amino acid, fluorescent protein, phosphine, genetic code expansion
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27368.55

構造登録者

Hu, C.,Duan, H.Z.,Liu, X.H.,Chen, Y.X.,Wang, J.Y. (登録日: 2022-07-06, 公開日: 2023-07-26, 最終更新日: 2026-03-04)

主引用文献

Duan, H.Z.,Hu, C.,Li, Y.L.,Wang, S.H.,Xia, Y.,Liu, X.,Wang, J.,Chen, Y.X.
Genetically Encoded Phosphine Ligand for Metalloprotein Design.
J.Am.Chem.Soc., 144:22831-22837, 2022

PubMed Abstract: Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins.

PubMed: 36417425
DOI: 10.1021/jacs.2c09683

実験手法

X-RAY DIFFRACTION (1.95 Å)