7Y86

CcpS mutant

7Y86 の概要

• エントリーDOI: 10.2210/pdb7y86/pdb
• 分子名称: UPF0297 protein A7J08_00425 (2 entities in total)
• 機能のキーワード: ccps, mutant, gene regulation
• 由来する生物種: Streptococcus suis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20999.34

構造登録者

Tang, J.S.,Ran, T.T.,Wang, W.W.,Fan, H.J. (登録日: 2022-06-22, 公開日: 2023-05-10, 最終更新日: 2023-11-29)

主引用文献

Tang, J.,Guo, M.,Chen, M.,Xu, B.,Ran, T.,Wang, W.,Ma, Z.,Lin, H.,Fan, H.
A link between STK signalling and capsular polysaccharide synthesis in Streptococcus suis.
Nat Commun, 14:2480-2480, 2023

PubMed Abstract: Synthesis of capsular polysaccharide (CPS), an important virulence factor of pathogenic bacteria, is modulated by the CpsBCD phosphoregulatory system in Streptococcus. Serine/threonine kinases (STKs, e.g. Stk1) can also regulate CPS synthesis, but the underlying mechanisms are unclear. Here, we identify a protein (CcpS) that is phosphorylated by Stk1 and modulates the activity of phosphatase CpsB in Streptococcus suis, thus linking Stk1 to CPS synthesis. The crystal structure of CcpS shows an intrinsically disordered region at its N-terminus, including two threonine residues that are phosphorylated by Stk1. The activity of phosphatase CpsB is inhibited when bound to non-phosphorylated CcpS. Thus, CcpS modulates the activity of phosphatase CpsB thereby altering CpsD phosphorylation, which in turn modulates the expression of the Wzx-Wzy pathway and thus CPS production.

PubMed: 37120581
DOI: 10.1038/s41467-023-38210-4

実験手法

X-RAY DIFFRACTION (1.5 Å)