7Y7O

Crystal structure of metallo-endoribonuclease YbeY from Staphylococcus aureus

7Y7O の概要

• エントリーDOI: 10.2210/pdb7y7o/pdb
• 分子名称: Endoribonuclease YbeY, ZINC ION, CITRIC ACID, ... (4 entities in total)
• 機能のキーワード: rna, endoribonuclease, metalloprotein, zinc, hydrolase
• 由来する生物種: Staphylococcus aureus subsp. aureus Mu50
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19321.46

構造登録者

Lee, J.,Ha, N.-C. (登録日: 2022-06-22, 公開日: 2023-03-08, 最終更新日: 2024-05-29)

主引用文献

Lee, J.,Jo, I.,Kwon, A.R.,Ha, N.C.
Crystal Structure of the Metallo-Endoribonuclease YbeY from Staphylococcus aureus.
J Microbiol Biotechnol., 33:28-34, 2023

PubMed Abstract: Endoribonuclease YbeY is specific to the single-stranded RNA of ribosomal RNAs and small RNAs. This enzyme is essential for the maturation and quality control of ribosomal RNA in a wide range of bacteria and for virulence in some pathogenic bacteria. In this study, we determined the crystal structure of YbeY from at a resolution of 1.9 Å in the presence of zinc chloride. The structure showed a zinc ion at the active site and two molecules of tricarboxylic acid citrate, which were also derived from the crystallization conditions. Our structure showed the zinc ion-bound local environment at the molecular level for the first time. Molecular comparisons were performed between the carboxylic moieties of citrate and the phosphate moiety of the RNA backbone, and a model of YbeY in complex with a single strand of RNA was subsequently constructed. Our findings provide molecular insights into how the YbeY enzyme recognizes single-stranded RNA in bacteria.

PubMed: 36457189
DOI: 10.4014/jmb.2209.09019

実験手法

X-RAY DIFFRACTION (1.9 Å)