7Y77

Crystal structure of rice NAL1

7Y77 の概要

• エントリーDOI: 10.2210/pdb7y77/pdb
• 分子名称: Protein NARROW LEAF 1, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: serine protease, nal1, hydrolase
• 由来する生物種: Oryza sativa (Asian cultivated rice)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 144058.74

構造登録者

Yan, J.J.,Guan, Z.Y.,Yin, P.,Xiong, L.Z. (登録日: 2022-06-21, 公開日: 2023-07-12, 最終更新日: 2024-05-29)

主引用文献

Li, W.,Yan, J.,Zhang, Y.,Zhang, F.,Guan, Z.,Yao, Y.,Chang, Y.,Tu, H.,Li, X.,Wang, H.,Xiong, H.,Lai, X.,Yin, P.,Xiong, L.
Serine protease NAL1 exerts pleiotropic functions through degradation of TOPLESS-related corepressor in rice.
Nat.Plants, 9:1130-1142, 2023

PubMed Abstract: NARROW LEAF 1 (NAL1) is a breeding-valuable pleiotropic gene that affects multiple agronomic traits in rice, although the molecular mechanism is largely unclear. Here, we report that NAL1 is a serine protease and displays a novel hexameric structure consisting of two ATP-mediated doughnut-shaped trimeric complexes. Moreover, we identified TOPLESS-related corepressor OsTPR2 involved in multiple growth and development processes as the substrate of NAL1. We found that NAL1 degraded OsTPR2, thus modulating the expression of downstream genes related to hormone signalling pathways, eventually achieving its pleiotropic physiological function. An elite allele, NAL1, which may have originated from wild rice, could increase grain yield. Furthermore, the NAL1 homologues in different crops have a similar pleiotropic function to NAL1. Our study uncovers a NAL1-OsTPR2 regulatory module and provides gene resources for the design of high-yield crops.

PubMed: 37349549
DOI: 10.1038/s41477-023-01449-2

実験手法

X-RAY DIFFRACTION (2.6 Å)