7Y5E

In situ single-PBS-PSII-PSI-LHCs megacomplex.

これはPDB形式変換不可エントリーです。

7Y5E の概要

• エントリーDOI: 10.2210/pdb7y5e/pdb
• EMDBエントリー: 33558 33561 33597 33605 33618
• 分子名称: Phycoerythrin alpha subunit, C-phycocyanin beta subunit, (2S)-2,3-dihydroxypropyl octadecanoate, ... (104 entities in total)
• 機能のキーワード: complex, photosynthesis
• 由来する生物種: Porphyridium purpureum; 詳細
• タンパク質・核酸の鎖数: 894
• 化学式量合計: 18653194.67

構造登録者

You, X.,Zhang, X.,Cheng, J.,Xiao, Y.N.,Sui, S.F. (登録日: 2022-06-17, 公開日: 2023-02-01, 最終更新日: 2024-07-03)

主引用文献

You, X.,Zhang, X.,Cheng, J.,Xiao, Y.,Ma, J.,Sun, S.,Zhang, X.,Wang, H.W.,Sui, S.F.
In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex.
Nature, 616:199-206, 2023

PubMed Abstract: In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs). Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS-PSII-PSI-LHC megacomplexes from the red alga Porphyridium purpureum at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS-PSII-PSI-LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI.

PubMed: 36922595
DOI: 10.1038/s41586-023-05831-0

実験手法

ELECTRON MICROSCOPY (3.3 Å)