7XQ5

Crystal structure of ScIno2p-ScIno4p bound promoter DNA

7XQ5 の概要

• エントリーDOI: 10.2210/pdb7xq5/pdb
• 分子名称: Protein INO4, Protein INO2, DNA (5'-D(P*CP*CP*TP*GP*CP*AP*TP*GP*TP*GP*AP*AP*AP*AP*T)-3'), ... (6 entities in total)
• 機能のキーワード: transcriptional regulators, transcription
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27175.12

構造登録者

Khan, M.H.,Lu, X. (登録日: 2022-05-06, 公開日: 2022-07-20, 最終更新日: 2023-11-29)

主引用文献

Khan, M.H.,Xue, L.,Yue, J.,Schuller, H.J.,Zhu, Z.,Niu, L.
Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: Gene expression is mediated by a series of regulatory proteins, i.e., transcription factors. Under different growth conditions, the transcriptional regulation of structural genes is associated with the recognition of specific regulatory elements (REs) in promoter DNA. The manner by which transcription factors recognize distinctive REs is a key question in structural biology. Previous research has demonstrated that Ino2p/Ino4p heterodimer is associated with the transcriptional regulation of phospholipid biosynthetic genes. Mechanistically, Ino2p/Ino4p could specifically recognize the inositol/choline-responsive element (ICRE), followed by the transcription activation of the phospholipid biosynthetic gene. While the promoter DNA sequence for Ino2p has already been characterized, the structural basis for the mutual interaction between Ino2p/Ino4p and their binding interface with promoter DNA remain relatively unexplored. Here, we have determined the crystalline structure of the Ino2p/Ino4p/DNA ternary complex, which highlights some residues (Ino2p and Ino4p) associated with the sequence-specific recognition of promoter DNA. Our biochemical analysis showed that mutating these residues could completely abolish protein-DNA interaction. Despite the requirement of Ino2p and Ino4p for interprotein-DNA interaction, both proteins can still interact-even in the absence of DNA. Combined with the structural analysis, our in vitro binding analysis demonstrated that residues (Arg35, Asn65, and Gln69 of Ino2p and Leu59 of Ino4p) are critical for interprotein interactions. Together, these results have led to the conclusion that these residues are critical to establishing interprotein-DNA and protein-DNA mutual interactions.

PubMed: 35886947
DOI: 10.3390/ijms23147600

実験手法

X-RAY DIFFRACTION (2.25 Å)