7X7N

3D model of the 3-RBD up single trimeric spike protein of SARS-CoV2 in the presence of synthetic peptide SIH-5.

7X7N の概要

• エントリーDOI: 10.2210/pdb7x7n/pdb
• EMDBエントリー: 33042
• 分子名称: Spike glycoprotein, Synthetic peptide SIH-5, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: spike protein, sars-cov2, complex, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 463757.97

構造登録者

Khatri, B.,Pramanick, I.,Malladi, S.K.,Rajmani, R.S.,Kumar, S.,Ghosh, P.,Sengupta, N.,Rahisuddin, R.,Kumaran, S.,Ringe, R.P.,Varadarajan, R.,Dutta, S.,Chatterjee, J. (登録日: 2022-03-10, 公開日: 2022-04-27, 最終更新日: 2025-07-02)

主引用文献

Khatri, B.,Pramanick, I.,Malladi, S.K.,Rajmani, R.S.,Kumar, S.,Ghosh, P.,Sengupta, N.,Rahisuddin, R.,Kumar, N.,Kumaran, S.,Ringe, R.P.,Varadarajan, R.,Dutta, S.,Chatterjee, J.
A dimeric proteomimetic prevents SARS-CoV-2 infection by dimerizing the spike protein.
Nat.Chem.Biol., 18:1046-1055, 2022

PubMed Abstract: Protein tertiary structure mimetics are valuable tools to target large protein-protein interaction interfaces. Here, we demonstrate a strategy for designing dimeric helix-hairpin motifs from a previously reported three-helix-bundle miniprotein that targets the receptor-binding domain (RBD) of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Through truncation of the third helix and optimization of the interhelical loop residues of the miniprotein, we developed a thermostable dimeric helix-hairpin. The dimeric four-helix bundle competes with the human angiotensin-converting enzyme 2 (ACE2) in binding to RBD with 2:2 stoichiometry. Cryogenic-electron microscopy revealed the formation of dimeric spike ectodomain trimer by the four-helix bundle, where all the three RBDs from either spike protein are attached head-to-head in an open conformation, revealing a novel mechanism for virus neutralization. The proteomimetic protects hamsters from high dose viral challenge with replicative SARS-CoV-2 viruses, demonstrating the promise of this class of peptides that inhibit protein-protein interaction through target dimerization.

PubMed: 35654847
DOI: 10.1038/s41589-022-01060-0

実験手法

ELECTRON MICROSCOPY (4.47 Å)