7X1M

The complex structure of Omicron BA.1 RBD with BD604, S309,and S304

7X1M の概要

• エントリーDOI: 10.2210/pdb7x1m/pdb
• EMDBエントリー: 32944
• 分子名称: S309 Fab heavy chain, S309 Fab light chain, BD-604 Fab heavy chain, ... (8 entities in total)
• 機能のキーワード: antibody viral protein complex, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 165881.03

構造登録者

Huang, M.,Xie, Y.F.,Qi, J.X. (登録日: 2022-02-24, 公開日: 2022-07-06, 最終更新日: 2025-07-02)

主引用文献

Huang, M.,Wu, L.,Zheng, A.,Xie, Y.,He, Q.,Rong, X.,Han, P.,Du, P.,Han, P.,Zhang, Z.,Zhao, R.,Jia, Y.,Li, L.,Bai, B.,Hu, Z.,Hu, S.,Niu, S.,Hu, Y.,Liu, H.,Liu, B.,Cui, K.,Li, W.,Zhao, X.,Liu, K.,Qi, J.,Wang, Q.,Gao, G.F.
Atlas of currently available human neutralizing antibodies against SARS-CoV-2 and escape by Omicron sub-variants BA.1/BA.1.1/BA.2/BA.3.
Immunity, 55:1501-1514.e3, 2022

PubMed Abstract: SARS-CoV-2 Omicron variant has presented significant challenges to current antibodies and vaccines. Herein, we systematically compared the efficacy of 50 human monoclonal antibodies (mAbs), covering the seven identified epitope classes of the SARS-CoV-2 RBD, against Omicron sub-variants BA.1, BA.1.1, BA.2, and BA.3. Binding and pseudovirus-based neutralizing assays revealed that 37 of the 50 mAbs lost neutralizing activities, whereas the others displayed variably decreased activities against the four Omicron sub-variants. BA.2 was found to be more sensitive to RBD-5 antibodies than the other sub-variants. Furthermore, a quaternary complex structure of BA.1 RBD with three mAbs showing different neutralizing potencies against Omicron provided a basis for understanding the immune evasion of Omicron sub-variants and revealed the lack of G446S mutation accounting for the sensitivity of BA.2 to RBD-5 mAbs. Our results may guide the application of the available mAbs and facilitate the development of universal therapeutic antibodies and vaccines against COVID-19.

PubMed: 35777362
DOI: 10.1016/j.immuni.2022.06.005

実験手法

ELECTRON MICROSCOPY (2.74 Å)