7WZ5

Larimichthys crocea IFNi

7WZ5 の概要

• エントリーDOI: 10.2210/pdb7wz5/pdb
• 分子名称: Interferon C, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: interferon, antiviral, cytokine
• 由来する生物種: Larimichthys crocea (large yellow croaker)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18196.29

構造登録者

Chen, J.J. (登録日: 2022-02-17, 公開日: 2023-02-08, 最終更新日: 2025-04-02)

主引用文献

Guan, Y.,Chen, J.,Guan, H.,Chen, T.T.,Teng, Y.,Wei, Z.,Li, Z.,Ouyang, S.,Chen, X.
Structural and Functional Characterization of a Fish Type I Subgroup d IFN Reveals Its Binding to Receptors.
J Immunol., 212:1207-1220, 2024

PubMed Abstract: Teleost fish type I IFNs and the associated receptors from the cytokine receptor family B (CRFB) are characterized by remarkable diversity and complexity. How the fish type I IFNs bind to their receptors is still not fully understood. In this study, we demonstrate that CRFB1 and CRFB5 constitute the receptor pair through which type I subgroup d IFN (IFNd) from large yellow croaker, Larimichthys crocea, activates the conserved JAK-STAT signaling pathway as a part of the antiviral response. Our data suggest that L. crocea IFNd (LcIFNd) has a higher binding affinity with L. crocea CRFB5 (LcCRFB5) than with LcCRFB1. Furthermore, we report the crystal structure of LcIFNd at a 1.49-Å resolution and construct structural models of LcIFNd in binary complexes with predicted structures of extracellular regions of LcCRFB1 and LcCRFB5, respectively. Despite striking similarities in overall architectures of LcIFNd and its ortholog human IFN-ω, the receptor binding patterns between LcIFNd and its receptors show that teleost and mammalian type I IFNs may have differentially selected helices that bind to their homologous receptors. Correspondingly, key residues mediating binding of LcIFNd to LcCRFB1 and LcCRFB5 are largely distinct from the receptor-interacting residues in other fish and mammalian type I IFNs. Our findings reveal a ligand/receptor complex binding mechanism of IFNd in teleost fish, thus providing new insights into the function and evolution of type I IFNs.

PubMed: 38345351
DOI: 10.4049/jimmunol.2300651

実験手法

X-RAY DIFFRACTION (1.39 Å)