7WW3

Crystal structure of MmIMP1-KH34 tandem domain

7WW3 の概要

• エントリーDOI: 10.2210/pdb7ww3/pdb
• 分子名称: Insulin-like growth factor 2 mRNA-binding protein 1 (2 entities in total)
• 機能のキーワード: imp1, igf2bp1, zbp1, rna binding, rbp, splicing, rna binding protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19654.71

構造登録者

Li, X.J.,Wu, B.X. (登録日: 2022-02-12, 公開日: 2023-02-22, 最終更新日: 2025-03-05)

主引用文献

Li, X.,Guo, W.,Wen, Y.,Meng, C.,Zhang, Q.,Chen, H.,Zhao, X.,Wu, B.
Structural basis for the RNA binding properties of mouse IGF2BP3.
Structure, 2025

PubMed Abstract: IGF2BP family proteins (IGF2BPs) contain six tandem RNA-binding domains (RBDs), resulting in highly complex RNA binding properties. Dissecting how IGF2BPs recognize their RNA targets is essential for understanding their regulatory roles in gene expression. Here, we have determined the crystal structures of mouse IGF2BP3 constructs complexed with different RNA substrates. Our structures reveal that the IGF2BP3-RRM12 domains can recognize CA-rich elements up to 5-nt in length, mainly through RRM1. We also captured the antiparallel RNA-binding mode of the IGF2BP3-KH12 domains, with five nucleotides bound by KH1 and two nucleotides bound by KH2. Furthermore, our structural and biochemical studies suggest that the IGF2BP3-KH12 domains could recognize the "zipcode" RNA element within the β-actin mRNA. Finally, we analyzed the similarities and differences of the RNA-binding properties between the KH12 and KH34. Our studies provide structural insights into RNA target recognition by mouse IGF2BP3.

PubMed: 39986276
DOI: 10.1016/j.str.2025.01.022

実験手法

X-RAY DIFFRACTION (1.9 Å)