7WTM

Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Dis-E

これはPDB形式変換不可エントリーです。

7WTM の概要

• エントリーDOI: 10.2210/pdb7wtm/pdb
• EMDBエントリー: 32791
• 分子名称: 18S rRNA, 40S ribosomal protein S14-A, 40S ribosomal protein S22-A, ... (18 entities in total)
• 機能のキーワード: ribosome biogenesis, 40s ribosome, ribosome
• 由来する生物種: Saccharomyces cerevisiae; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 909744.27

構造登録者

Cheng, J.,La Venuta, G.,Lau, B.,Berninghausen, O.,Beckmann, R.,Hurt, E. (登録日: 2022-02-05, 公開日: 2022-10-19, 最終更新日: 2024-06-26)

主引用文献

Cheng, J.,La Venuta, G.,Lau, B.,Berninghausen, O.,Beckmann, R.,Hurt, E.
In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation.
Nucleic Acids Res., 50:11916-11923, 2022

PubMed Abstract: The transition of the 90S to the pre-40S pre-ribosome is a decisive step in eukaryotic small subunit biogenesis leading to a first pre-40S intermediate (state Dis-C or primordial pre-40S), where the U3 snoRNA keeps the nascent 18S rRNA locally immature. We in vitro reconstitute the ATP-dependent U3 release from this particle, catalyzed by the helicase Dhr1, and follow this process by cryo-EM revealing two successive pre-40S intermediates, Dis-D and Dis-E. The latter has lost not only U3 but all residual 90S factors including the GTPase Bms1. In vitro remodeling likewise induced the formation of the central pseudoknot, a universally conserved tertiary RNA structure that comprises the core of the small subunit decoding center. Thus, we could structurally reveal a key tertiary RNA folding step that is essential to form the active 40S subunit.

PubMed: 36263816
DOI: 10.1093/nar/gkac910

実験手法

ELECTRON MICROSCOPY (3.5 Å)