7WM4

Cryo-EM structure of tetrameric TLR3 in complex with dsRNA (90 bp)

7WM4 の概要

• エントリーDOI: 10.2210/pdb7wm4/pdb
• EMDBエントリー: 32599
• 分子名称: Toll-like receptor 3, RNA (81-MER), beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: innate immunity, immune system, immune system-rna complex, immune system/rna
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 377646.32

構造登録者

Sakaniwa, K.,Ohto, U.,Shimizu, T. (登録日: 2022-01-14, 公開日: 2023-01-25, 最終更新日: 2025-07-02)

主引用文献

Sakaniwa, K.,Fujimura, A.,Shibata, T.,Shigematsu, H.,Ekimoto, T.,Yamamoto, M.,Ikeguchi, M.,Miyake, K.,Ohto, U.,Shimizu, T.
TLR3 forms a laterally aligned multimeric complex along double-stranded RNA for efficient signal transduction.
Nat Commun, 14:164-164, 2023

PubMed Abstract: Toll-like receptor 3 (TLR3) is a member of the TLR family, which plays an important role in the innate immune system and is responsible for recognizing viral double-stranded RNA (dsRNA). Previous biochemical and structural studies have revealed that a minimum length of approximately 40-50 base pairs of dsRNA is necessary for TLR3 binding and dimerization. However, efficient TLR3 activation requires longer dsRNA and the molecular mechanism underlying its dsRNA length-dependent activation remains unknown. Here, we report cryo-electron microscopy analyses of TLR3 complexed with longer dsRNA. TLR3 dimers laterally form a higher multimeric complex along dsRNA, providing the basis for cooperative binding and efficient signal transduction.

PubMed: 36631495
DOI: 10.1038/s41467-023-35844-2

実験手法

ELECTRON MICROSCOPY (3.2 Å)