7WLM

The Cryo-EM structure of siphonaxanthin chlorophyll a/b type light-harvesting complex II

Summary for 7WLM

• Entry DOI: 10.2210/pdb7wlm/pdb
• EMDB information: 32588
• Descriptor: siphonaxanthin chlorophyll a/b binding light-harvesting complex II, CHLOROPHYLL B, CHLOROPHYLL A, ... (8 entities in total)
• Functional Keywords: light-harvesting complex ii, siphonaxanthin, chlorophyll b replacement, blue-green utilization, photosynthesis
• Biological source: Codium fragile
• Total number of polymer chains: 3
• Total formula weight: 115869.57

Authors

Seki, S.,Nakaniwa, T.,Castro-Hartmann, P.,Sader, K.,Kawamoto, A.,Tanaka, H.,Qian, P.,Kurisu, G.,Fujii, R. (deposition date: 2022-01-13, release date: 2022-11-23, Last modification date: 2024-06-26)

Primary citation

Seki, S.,Nakaniwa, T.,Castro-Hartmann, P.,Sader, K.,Kawamoto, A.,Tanaka, H.,Qian, P.,Kurisu, G.,Fujii, R.
Structural insights into blue-green light utilization by marine green algal light harvesting complex II at 2.78 angstrom.
Bba Adv, 2:100064-100064, 2022

PubMed Abstract: Light-harvesting complex II (LHCII) present in plants and green algae absorbs solar energy to promote photochemical reactions. A marine green macroalga, , exhibits the unique characteristic of absorbing blue-green light from the sun during photochemical reactions while being underwater owing to the presence of pigment-altered LHCII called siphonaxanthin-chlorophyll binding protein (SCP). In this study, we determined the structure of SCP at a resolution of 2.78 Å using cryogenic electron microscopy. SCP has a trimeric structure, wherein each monomer containing two lutein and two chlorophyll molecules in the plant-type LHCII are replaced by siphonaxanthin and its ester and two chlorophyll molecules, respectively. Siphonaxanthin occupies the binding site in SCP having a polarity in the trimeric inner core, and exhibits a distorted conjugated chain comprising a carbonyl group hydrogen bonded to a cysteine residue of apoprotein. These features suggest that the siphonaxanthin molecule is responsible for the characteristic green absorption of SCP. The replaced chlorophyll molecules extend the region of the stromal side chlorophyll cluster, spanning two adjacent monomers.

PubMed: 37082593
DOI: 10.1016/j.bbadva.2022.100064

Experimental method

ELECTRON MICROSCOPY (2.8 Å)