7WKO

ICP1 Csy1-2 complex

7WKO の概要

• エントリーDOI: 10.2210/pdb7wko/pdb
• 分子名称: Csy1, Csy2 (3 entities in total)
• 機能のキーワード: crispr-cas, phage icp1, rna binding protein
• 由来する生物種: Vibrio phage ICP1_2011_A; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52674.64

構造登録者

Zhang, M.,Peng, R. (登録日: 2022-01-10, 公開日: 2023-04-26, 最終更新日: 2023-11-29)

主引用文献

Zhang, M.,Peng, R.,Peng, Q.,Liu, S.,Li, Z.,Zhang, Y.,Song, H.,Yang, J.,Xing, X.,Wang, P.,Qi, J.,Gao, G.F.
Mechanistic insights into DNA binding and cleavage by a compact type I-F CRISPR-Cas system in bacteriophage.
Proc.Natl.Acad.Sci.USA, 120:e2215098120-e2215098120, 2023

PubMed Abstract: CRISPR-Cas systems are widespread adaptive antiviral systems used in prokaryotes. Some phages, in turn, although have small genomes can economize the use of genetic space to encode compact or incomplete CRISPR-Cas systems to inhibit the host and establish infection. Phage ICP1, infecting , encodes a compact type I-F CRISPR-Cas system to suppress the antiphage mobile genetic element in the host genome. However, the mechanism by which this compact system recognizes the target DNA and executes interference remains elusive. Here, we present the electron cryo-microscopy (cryo-EM) structures of both apo- and DNA-bound ICP1 surveillance complexes (Aka Csy complex). Unlike most other type I surveillance complexes, the ICP1 Csy complex lacks the Cas11 subunit or a structurally homologous domain, which is crucial for dsDNA binding and Cas3 activation in other type I CRISPR-Cas systems. Structural and functional analyses revealed that the compact ICP1 Csy complex alone is inefficient in binding to dsDNA targets, presumably stalled at a partial R-loop conformation. The presence of Cas2/3 facilitates dsDNA binding and allows effective dsDNA target cleavage. Additionally, we found that Cas2/3 efficiently cleaved the dsDNA target presented by the ICP1 Csy complex, but not vice versa. These findings suggest a unique mechanism for target dsDNA binding and cleavage by the compact phage-derived CRISPR-Cas system.

PubMed: 37094126
DOI: 10.1073/pnas.2215098120

実験手法

X-RAY DIFFRACTION (2.3 Å)