7WH9

holo structure of emodin 1-OH O-methyltransferase complex with emodin and S-Adenosyl-L-homocysteine

7WH9 の概要

• エントリーDOI: 10.2210/pdb7wh9/pdb
• 分子名称: O-methyltransferase gedA, 3-METHYL-1,6,8-TRIHYDROXYANTHRAQUINONE, S-ADENOSYL-L-HOMOCYSTEINE, ... (4 entities in total)
• 機能のキーワード: o-methyltransferase, emodin, transferase
• 由来する生物種: Aspergillus terreus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 170082.25

構造登録者

Liang, Y.J.,Lu, X.F.,Qi, F.F.,Xue, Y.Y. (登録日: 2021-12-30, 公開日: 2023-01-11, 最終更新日: 2024-05-29)

主引用文献

Xue, Y.,Liang, Y.,Zhang, W.,Geng, C.,Feng, D.,Huang, X.,Dong, S.,Zhang, Y.,Sun, J.,Qi, F.,Lu, X.
Characterization and Structural Analysis of Emodin- O -Methyltransferase from Aspergillus terreus.
J.Agric.Food Chem., 70:5728-5737, 2022

PubMed Abstract: All -methylated derivatives of emodin, including physcion, questin, and 1--methylemodin, show potential antifungal activities. Notably, emodin and questin are two pivotal intermediates of geodin biosynthesis in . Although most of the geodin biosynthetic steps have been investigated, the key -methyltransferase (OMT) responsible for the -methylation of emodin to generate questin has remained unidentified. Herein, through phylogenetic tree analysis and biochemical assays, the long-sought class II emodin--methyltransferase GedA has been functionally characterized. Additionally, the catalytic mechanism and key residues at the catalytic site of GedA were elucidated by enzyme-substrate-methyl donor analogue ternary complex crystal structure determination and site-directed mutagenesis. As we demonstrate, GedA adopts a typical general acid/base (E446/H373)-mediated transmethylation mechanism. In particular, residue D374 is also crucial for efficient catalysis through blocking the formation of intramolecular hydrogen bonds in emodin. This study will facilitate future engineering of GedA for the production of physcion or other site-specific -methylated anthraquinone derivatives with potential applications as biopesticides.

PubMed: 35475366
DOI: 10.1021/acs.jafc.2c01281

実験手法

X-RAY DIFFRACTION (2.803 Å)